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ANNUAL REPORT
April 27, 2001 - June 30, 2002


SACKLER INSTITUTE FOR DEVELOPMENTAL PSYCHOBIOLOGY
AT COLUMBIA UNIVERSITY


The Sackler Institute and Professorship were funded at Columbia in December 2000 by a gift from the Sackler Foundation and formally established by act of the Columbia University Faculty Senate on April 27th 2001. This report covers the first full year of operations through the end of the fiscal year, June 30, 2002. The Sackler Award program, established in 1998, became a part of the Sackler Institute at the time of its founding.

The structure and faculty of the Institute during this first year were as follows:

Director, Dr. Myron A. Hofer
Assistant Director, Dr. William P. Fifer
Chief, Basic Science Division - Dr. Thomas Jessell
Chiefs, Behavioral Neuroscience Division - Drs. Michael Myers and William Fifer
Chief, Clinical Division - Dr. Myrna Weissman
Head, Developmental Neuroimaging Laboratory - Dr. Bradley Peterson
Liason, Cornell - Sackler Institute - Dr. Jonathan Polan
Sackler Awardees: 2000-2001, Dr. Catherine Monk
2002-2003, Drs. Joshua Gordon and Cheryl Corcoran


Construction of the Sackler Laboratories moved forward swiftly and was completed in October 2001. The New York State Psychiatric Institute Department of Developmental Psychobiology moved into the offices and laboratories in November and a tour of the space took place after the first meeting of the Internal Advisory Committee on November 28th, 2001. Dr. Mortimer Sackler visited the new laboratories and met informally with the faculty on January 22, 2002. The offices, laboratories and conference room are working out extremely well and the Sackler faculty that have moved into the laboratories are delighted with them. This space has already become an important geographic center for the Institute's activities at the medical center.

Faculty and Sackler Awardees
A new faculty member was appointed as a Sackler Scientist in July of 2001. Dr. Bradley Peterson was recruited from Yale, and heads up the new Developmental Neuroimaging Laboratory in the Institute. Dr. Peterson has an international reputation in functional magnetic imaging of childhood psychiatric disorders and leads a multi-site study collecting serial MRI data on a large cohort of several hundred children from the neonatal period forward. Dr. Peterson's research is further described in the Faculty Research section that follows.

Dr. Thomas Jessell, was awarded the 13th Annual Bristol Myers Squibb Award for distinguished achievement in neuroscience in September 2001.

The first Sackler Awardee, Dr. Catherine Monk, was awarded a 5 year Research Scientist Development Award from NIMH to continue her research on fetal origins of vulnerability to anxiety and depression and the role of maternal to fetal transmission of physiological signs of anxiety. Clearly the Sackler Award played a crucial role in her career, making the highly competitive grant possible. Dr. Monk's report on her Sackler Award research is included as the final section of this report.

The timely award of federal funding to Dr. Monk opened the Sackler Award Program to new applicants. We received 7 excellent applications and deciding between them was extremely difficult. In the end, the Award Committee decided to fund two of the applicants by extending the start date 2 months and using the remainder of the funds made available as a result of Dr. Monks' federal grant award.

Dr. Joshua Gordon will be studying the novel early developmental effects of an inducible, region-specific gene knockout of the serotonin 1A receptor. This new advance in molecular genetic technique allows the investigator to control expression of the targeted gene, turning it on or off at any desired developmental stage. Dr. Gordon's neurophysiological studies will be done in the Department of Neurobiology and Behavior as well as the Department of Psychiatry.

Dr. Cheryl Corcoran's project involves the prospective study of how Schizophrenia is triggered in an unusual population of adolescents at very high risk (50% in 5years) due to prodromal symptoms and/or a very strong family history. Salivary cortisol has been found to be a novel predictor in Dr. Corcoran's preliminary work.

Sackler Fellows

Through the Columbia contribution to the Institute, we were able to appoint 3 Sackler fellows, two of whom received $5,000.00 mini-grants (Drs. Schechter and Feder) and one is being supported by our NIMH funded Research Training Program (Dr. Inge-Marie Eigsti). Dr. Schechter, working with Drs. Hofer and Myers, has been studying the intergenerational transmission of trauma in high risk populations of mothers with past histories of abuse, and their infants. Dr. Schechter has been invited to present his findings at the World Association for Mental Health, the American Psychiatric Association and the International Society of Traumatic Stress. Dr. Feder has been working with Dr. Weissman studying the familial transmission of depression across 3 generations, searching for markers of latent genetic risk using quantitative EEG and startle response measures. Dr. Eigsti is the first joint Columbia-Cornell Sackler Institute Fellow and will be studying the deployment of attention in autistic and normal children using novel perceptual tests and concurrent neuro-imaging. She will be working with Dr. Fifer here at Columbia and Dr. Casey at Cornell.

Interdisciplinary Conferences

Other activities included our joint sponsorship, with the Parent-Infant Program of the Columbia Psychoanalytic Institute, of a National Conference on November 3rd 2001 with the title " When the Bough Breaks: Attachment Theory, Psychobiology and Social Policy". Drs. Myron Hofer, Bradley Peterson and Daniel Schechter were among the speakers as well as Dr. Susan Coates of the Columbia Parent - Infant Program. The meeting plans were modified after September 11th to include Dr. Robert Pynoos, an authority on Childhood Traumatic Stress and a panel discussion on Public Policy chaired by Dr. Herbert Pardes. The conference was well attended and generated a great deal of subsequent interest, resulting in a book contract with the Analytic Press. Drs. Coates and Schechter are editing this book which will be the first major publication of the Sackler-funded Parent - Infant Program and the Sackler Institute at Columbia.

The Institute also sponsored two interdisciplinary symposia at international meetings: one on Autism at the annual meeting of the International Society for Developmental Psychobiology and the other on Early Learning and Attention at the Annual Winter Conference of Developmental Science. In addition, three visiting scientists were sponsored to come to Columbia to meet with Sackler Scientists to share their expertise in new research methods and approaches in the areas of: early cortisol regulation, visual motor development and infant eye blink conditioning - animal and human.

The Columbia Parent - Infant Program, established through funding from the Sackler Award in the years 1999-2000, sponsored a symposium at the 8th Congress World Association for Infant Mental Health Conference in Amsterdam this summer. This was very well received and in his discussion of Dr. Schechter's paper (see Sackler Fellows above) Dr. Peter Fonagy, one of the leading figures in this field, gave this assessment:

"The Columbia PIP group has arrived at that elusive Holy Grail of infant
mental health work, the deep and genuine integration of research, theory and clinical practice. There are but a handful of groups around the world that can lay claim to such an achievement. It is indeed a privilege to discuss this work and underscore for you the coherence and sophistication which reflects the integration of research and practice based on solid theoretical foundations."

Our plans for next year include a workshop series here at Columbia on Experimental Mutant Mice and on Gene Protein Chip Technology as well as a major conference on New Findings in Developmental Effects of Maternal Smoking.

Faculty Research

Brief reports on the research of each of the 7 Sackler Scientists over the past year are given in alphabetical order below. Their research, taken together, spans the full interdisciplinary range from clinical epidemiology to molecular genetics, as is presented below. Following this narrative, a listing of the publications over the past year and the active grant support of each scientist is given, again in alphabetical order. A report on the research of Sackler Awardee, Dr. Catherine Monk makes up the final section of this report.

1. Human Fetal Behavior and Intrauterine Influences on Vulnerability to Psychiatric Illness - Dr. William Fifer

The general research program has focused on the effects of the early environment on fetal and infant brain/behavior development. Our most recent efforts have focused on the effects of prenatal risk factors including, maternal nicotine and alcohol use during pregnancy, as well as maternal stress and anxiety, on autonomic nervous system development. With Dr. Monk, a former Sackler Fellow, and Dr. Sloan from the Department of Behavioral Medicine, we have expanded the research to include the influence of maternal depression and anxiety on fetal and infant development. Also with Dr. Myers we continue to study early markers of risk for Developmental Disorders and Sudden Infant Death (SIDS). This NICHD funded study involves a mutually beneficial technology exchange between New York State Psychiatric Institute and the high-risk populations in Washington Heights and on the Pine Ridge Reservation in South Dakota. A further extension of this work, with the Department of Pediatrics, focuses on the developing nervous system in prematurely born infants and, together with colleagues from the Division of Environmental Health Science at Columbia, assessments of sleep dependent physiology in infants exposed to environmental toxins during pregnancy.

During this past year we have also initiated three new studies in active
collaboration with colleagues from the Sackler Institute at Cornell. With B.J.
Casey we have designed a study to investigate the relationship between markers
of autonomic nervous system regulation in the perinatal period with attentional
regulation in older children. Inge-Marie Eigsti has begun work on a study of brain
activation and low-level issues of attention and learning in newborn human
infants using an eye blink conditioning paradigm, and with Bruce McCandliss, we
are exploring the use of high density EEG technology to investigate the ontogeny
of event related potentials in very young infants.


2. Biological Mechanisms in Early Separation Anxiety - Dr. Myron Hofer
Recent research with Drs. Susan Brunelli and Harry Shair has focused on early separation anxiety employing a novel developmental - genetic approach: selective breeding for high and low levels of separation calling in infant rats. This is the first study ever conducted on selective breeding for an infantile trait in a mammal, and raises the fascinating question of how continued selection can affect the developmental trajectory of the trait over time, both before and after the age at which selection is carried out. Since natural selection is known to act on infantile traits, this study brings evolution and its effects on development into the realm of laboratory study. Furthermore, it allows us to study the brain mechanisms responsible for extreme high and low levels of this first anxiety behavior, mechanisms that we and others have shown are very similar to those acted upon by known anxiolytic drugs and experimental anxiogenic compounds previously studied in humans. A recent publication, provided in the next section, describes some of the interesting questions raised by this study and the answers we are beginning to get from the data generated.

My other interest is in the theory of development and the implications of our work for new conceptualizations of attachment, separation anxiety and the long term developmental effects of different parenting patterns on adult vulnerability to psychiatric illness. Several of my papers on these subjects published in the past year are listed in the publicatons section.


3. Molecular Genetic Specifications of Developing Neuronal Circuitry
- Dr. Thomas Jessell

Our work has addressed the mechanisms that control the assembly of neural circuits in the vertebrate central nervous system. These studies have explored the link between neuronal identity and circuitry: how does the early specification of neuronal identity define the position of neurons, the projection pattern of their axons, and the selectivity of their synaptic contacts? We have focused on neurons that form the monosynaptic stretch reflex circuit in the spinal cord, for two reasons. First, this circuit forms early in development, in an activity-independent manner, suggesting that its assembly is genetically encoded. Second, a century of anatomy and physiology, from the pioneering studies of Sherrington and Ramón y Cajal onward, has provided a rigorous cellular and functional framework for interpreting molecular steps in the assembly sensory-motor connections. Over the past year we have addressed molecular basis of motor neuron projections into the periphery, and the central connections between proprioceptive sensory neurons and motor neurons.


4. Early Nutritional Influences on Vulnerability to Disease - Dr. Michael Myers

For the past several years, there has been renewed interest in the effects of inadequate nutrition during pregnancy, undergrowth of the fetus, and vulnerability to disease later in life. From large epidemiological studies, it appears that cardiovascular disease, diabetes, schizophrenia, and depression all share the common trait of being influenced by disturbances during this critical period of development. While long-term effects of early experiences has been a focus of much research within the field of Developmental Psychobiology, the proximal mechanisms and chain of events that account for enduring changes in disease vulnerability remain largely undiscovered. This has been a ongoing focus of work in my laboratory. This year, in collaboration with Drs. William Fifer and Harry Shair in our department, Dr. Robert Vosatka in Pediatrics at Columbia, and Drs. Morris Cohen and David Brown at Newark Beth Israel Hospital, we obtained a new five year NIH grant for over $2,000,000 to pursue this work in both animal models and human infants.

These new studies will focus on effects of variation in nutrient availability in the perinatal period on physiological, biochemical and behavioral characteristics of newborn infants. Indices derived from animal studies will be incorporated into human studies, and the underpinnings of correlative findings from human studies will be pursued in animal models. The studies will determine if human infants with low birth weights, or rats whose mothers were underfed during pregnancy, express differences in cardiovascular and behavioral responses to feeding and postural challenge. In addition, we are pursuing the hypothesis that alterations in methylation of regulatory regions of certain genes are key to understanding the permanent changes in gene expression that underlying the early effects of variation in growth.


5. Neuroimaging in Children At Risk - Dr. Bradley Peterson
The developmental neuroimaging laboratory successfully moved from Yale University to Columbia and the Psychiatric Institute. Key staff were retained and new staff were hired, including an Assistant Professor who is a skilled electrical engineer with a specialty in MRI signal processing.

Imaging studies of impulse control in normal and ill children and adults have continued. Scanning is still performed at Yale, and we hope to transition to scanning on a new scanner at the Psychiatric Institute that is scheduled for operation in the spring of 2003.

New collaborative research has begun with established investigators at Columbia. Most notably, anatomical and functional imaging studies have begun on a large, 3-generational cohort followed by Dr. Myrna Weissman over the past 20 years. Individuals in the 2nd and 3rd generation who are at either high or low risk for major depressive illness are being studied with MRI and with neuropsychological tasks designed to test the integrity of neural systems thought to be dysfunctional in depression. An R01 was submitted in the spring of 2002 to fund this study.

Imaging studies of autistic spectrum disorder have begun in collaboration with Dr. Marc Patterson, Chief of Pediatric Neurology at Columbia, Dr. Agnes Whitaker, Director of the Neuropsychiatry Clinic, and Dr. Ian Lipkin, Director of the Center for Immunopathogenesis and Infectious Diseases at Columbia.


6. Origins of the First Infant Attachment - Dr. Jonathan Polan

We have been studying the transformation of the newborn's first undirected movements into fully competent maternally-directed behaviors -- orienting behaviors by which the infant mammal achieves and maintains contact with its mother. We have discovered, to our surprise, that whereas newborn rat pups perform spontaneous and reflex orienting behaviors equally often with or without the mother, in the first hours after birth, over the next two days they come to reserve them exclusively for encounters with the mother’s specific features. In the same period, infants develop the first evidence of motivation: performing these behaviors more intensely after a period of her absence. Together, specificity for maternal features and maternal deprivation-induced enhancement equip the newborn rat with a robust preference for interaction with its mother, and are core attributes of an “attachment” to her. These behaviors, initially performed with little relation to each other, develop surprisingly rapidly into organized patterns and become functionally linked with the better known behaviors of suckling.

We are currently exploring three developmental questions raised by these early behavioral events: 1) What behavioral processes are responsible for such rapid development of deprivation-induced enhancement of maternally-directed orienting behaviors? 2) What processes of learning about the mother contribute to the newborn’s orienting behaviors gaining specificity for her features? 3) What behavioral mechanisms relate maternally-directed orienting behaviors to the nipple grasp and suckling?


7. Clinical and Epidemiological Studies of Genetic Risk and Biological Markers in the Development of Affective Disorders - Dr. Myrna Weissman

Myrna Weissman, Ph.D., Virginia Warner, M.P.H., and Priya Wickramaratne, Ph.D. are completing data collection on a three generation longitudinal study of depressed grandparents, their offspring and grandchildren. The study includes diagnostic and neuropsychological assessment across the generation. In collaboration with Bradley Peterson, M.D., an NIMH grant has been submitted to conduct fMRI studies in the second and third generation. We've just learned that this study received a favorable review and will be funded.
Myrna Weissman, Ph.D. and Dan Pilowsky, M.D. have initiated a 6 site study funded by NIMH to determine the impact of treating maternal depression on the children’s symptoms and functioning. Also, directly related to the Sackler Institute: Sanjay Mathew, M.D., Postdoctral Fellow, received an American Foundation for Suicide Prevention Young Investigator Award to conduct neuroimaging studies of brain structure in suicidal and non-suicidal depressed adolescents grown-up. Adriana Feder, M.D., Assistant Professor, received a NARSAD Junior Investigator Award for her project "The Neurobiology of Prepubertal Onset Depression" and is completing the data collection on the psychopathology in offspring of depressed mothers coming to primary care. Yoko Nomura, Ph.D., Research Associate, developed and submitted a study examining the psychosocial and biomedical causes and sequelae associated with perinatal risk, such as low birth weight and pre-term birth.





Faculty Publications (2001-2002)

WILLIAM FIFER
Fifer,W.P. and Myers, M.M. Sudden fetal and infant deaths: shared characteristics and distinctive features. Seminars in Perinatology, 26(1):89-96,2002.

Monk, C., Fifer, W.P., Sloan, R.P, Myers, M.M., Bagiella, E., Ellman, L. and Hurtado, A. Physiologic responses to cognitive challenge during pregnancy: Effects of task and repeat testing. International Journal of Psychophysiology, 40(2):149-159, 2001.

Fifer, W.P., Monk, C. and Grose-Fifer, J. Prenatal development & risk. In G.
Bremner and A. Fogel (Eds). Blackwell Handbook of Infancy Research, Oxford,
UK: Blackwell Publishers Ltd., 505-542, 2001.

MYRON HOFER
Hofer, M.A., Brunelli, S.A. and Shair, H.S. Developmental effects of selective breeding for an infantile trait: the rat pups ultrasonic isolation call (USV). Develop. Psychobiol. 39, 1-16, 2001.

Brunelli, S.A., Hofer, M.A. and Weller, A. Selective breeding for infant vocal response: role for postnatal maternal effects? Develop. Psychobiol. 38:221-228,2001.

Hofer, M.A. and Sullivan, R.M. Toward A Neurobiology of Attachment. In Nelson, C.A. and Luciana, M. (Eds.) Handbook of Developmental Cognitive Neuroscience, MIT Press, Boston, 599-616, 2001.

Hofer, M.A., Shair, H.S. and Brunelli, S.A. Ultrasonic vocalization in rat and mouse pups in Current Protocols in Neuroscience, 8.14.1 to 8.14.16, 2001.

Hofer, M.A. Origins of Attachment and Regulators of Development within Early Social Interactions: From Animal to Human. In Kalverboer, A.F. and Gramsbergen, A. (Eds) Handbook of Brain and Behaviour in Human Development, Kluwer Academic Publishers, Great Britain, 821-840, 2001

Hofer, M.A. Evolutionary concepts of anxiety. In Stein D.J. and Hollander, E. (Eds.) Textbook of Anxiety Disorders, Am. Psychiatric Press, Washington, 65-78, 2002.




THOMAS JESSELL
Pierani, A. L. Moran-Rivard, M.J. Sunshine, D.R. Littman, M. Goulding, and T.M. Jessell (2001). Control of interneuron fate in the developing spinal cord by the progenitor homeodomain protein Dbx1. Neuron. 29, 367-384.
Muhr, J., Andersson, E., Persson, M., Jessell, T.M. and Ericson, J. (2001). Groucho-mediated transcriptional repression establishes progenitor cell pattern and neuronal fate in the ventral neural tube. Cell, 104, 861-873.
Wilson, S.I., Rydstrom, A., Trimborn, T., Willert, K., Nusse, R., Jessell, T.M., Edlund, T. (2001). The status of Wnt signaling regulates neural and epidermal fates in the chick embryo. Nature, 411, 325-330.
Briscoe, J., Chen, Y., Jessell, T.M. and Struhl, G. (2001). A hedgehog-insensitive form of patched provides evidence for direct long-range patterning activity of Sonic hedgehog in the neural tube. Molecular Cell 7, 1279-1291.
Srinivas S, Watanabe T, Lin CS, William CM, Tanabe Y, Jessell TM, and Costantini F. (2001). Cre reporter strains produced by targeted insertion of EYFP and ECFP into the ROSA26 locus. BMC Dev Biol. 2001;1:4.
Yang, X, Arber, S., William, C., Li, L., Tanabe, Y., Jessell, T.M., Birchmeier, C., and Burden, S.T. (2001) Patterning of Muscle Acetylcholine Receptor Gene Expression in the Absence of Motor Innervation. Neuron, 30, 399-410.
Novitch, B.G., Chen, A.I., and Jessell, T.M. (2001). Coordinate regulation of motor neuron subtype identity and pan-neuronal properties by the bHLH repressor Olig2. Neuron, 31, 773-789.
Vallstedt, A., Muhr, J., Pattyn, A., Pierani, A., Mendelsohn, M., Sander, M., Jessell, T., and Ericson, J. (2001). Different levels of repressor activity assign redundant and specific roles to Nkx6 genes during motor neuron and interneuron specification. Neuron, 31, 743-755.
Liu, J.P., Laufer, E. and Jessell, T.M. (2001). Assigning the positional identity of spinal motor neurons: rostrocaudal patterning of Hox-c protein expression by FGFs, Gdf11 and Retinoids. Neuron, 32, 997-1012.
Price, S.R., De Marco Garcia, N.V. Ranscht, B. and Jessell, T.M. (2002). Regulation of motor neuron pool sorting by differential expression of type II cadherins. Cell, 109, 215-216.
Muller, T., Brohmann, H., Pierani, A., Heppenstall, P.A., Lewin, G.R., Jessell, T.M., and Birchmeier, C. (2002) The homeodomain factor Lbx1 defines two programs of dorsal horn neuron differentiation. Neuron, 34, 551-562.
Pun, S., Sigrist, M., Santos, A.F., Rüegg, Sanes, J.R., Jessell, T.M., Arber, S., and Caroni, P. (2002). An intrinsic distinction in neuromuscular junction assembly and maintenance in different skeletal mucles. Neuron, 34, 357-370.
Nordström, U., Jessell, T.M., and Edlund, T. (2002). Progressive induction of caudal neural character by graded Wnt signaling. Nature Neuroscience, 5, 525-532.
Livet, J., Sigrist, M., Stroebel, S., Price, S.R., Henderson, C.E., Jessell, T.M, and Arber S. (2002). ETS gene Pea3 regulates motor neuron position and axonal branching. Neuron, 35, 877-892.
Wichterle, H., Lieberam, I., Porter, J., and Jessell, T.M. (2002). Directed differentiation of embryonic stem cells into motor neurons. Cell, 110, 385-397.

MICHAEL MYERS
Cohen, M, Brown, DR and Myers MM. Cardiovascular responses to pacifier experience and feeding in newborn infants. Developmental Psychobiology, 39: 34-39, 2001.

Sloan, RP, Bagiella E, Shapiro PA, Kuhl JP, Chernikhova D, Berg J and Myers MM. Hostility, gender, and cardiac control. Pyschosomatic Medicine, 63: 434-440.

Monk C, Fifer WP, Sloan RP, Myers, MM, Bagiella E, Ellman L, and Hurtado A. Physiologic responses to cognitive challenge during pregnancy: effect of task and repeated testing. International Journal of Psychophysiology, 40: 149-159, 2001.

Fifer WP and Myers, MM. Sudden fetal and infant deaths: shared characteristics and distinctive features. Seminars in Perinatology, 26: 89-96, 2002.

BRADLEY PETERSON
Peterson BS, Ment LR. The study of preterm birth and developmental brain abnormalities. Trends Neurosci, 24: 131-132, 2001.

Peterson BS, Ment LR. The necessity and difficulty of conducting MRI studies on infant brain development. Pediatrics, 107:593-594, 2001.

Peterson BS, Feineigle PA, Staib LH, Gore JC. Automated measurement of latent morphological features of the human corpus callosum. Hum Brain Mapping, 12:232-245, 2001.

Anderson AW, Colson ER, Marois R, Peterson BS, Duncan CC, Ehrenkranz RA, Konstantino M, Sarofin H, Schneider KC, Gore JC, Ment LR. Neonatal auditory activation detected by functional magnetic resonance imaging. Magnet Reson Imaging, 19:1-5, 2001.

Peterson BS, Staib L, Scahill L, Zhang H, Anderson C, Leckman JF, King R, Gore JC, Cohen DJ, Albert J, Webster R. Regional brain and ventricular volumes in Tourette syndrome. Arch Gen Psychiatry, 58:427-440, 2001.

Jeong J, Gore JC, Peterson BS. Mutual information analysis of the EEG in patients with Alzheimer's disease. Clin Neurophys, 112: 827-835, 2001.

Peterson BS, Pine DS, Cohen P, Brook J. A prospective, longitudinal study of tic, obsessive-compulsive, and attention deficit-hyperactivity disorders in an epidemiological sample. J Amer Acad Child Adolesc Psychiatry, 40: 685-695, 2001.

Morshed SA, Parveen S, Leckman JF, Mercadante MT, Bittencourt-Kiss MH, Miguel EC, Yazgan Y, Fujii T, Paul S, Peterson BS, Zhang H, King RA, Scahill L, Lombroso PJ. Antibodies against striatal, nuclear, cytoskeletal and streptococcal epitopes in children and adults with Tourette’s syndrome, Sydenham’s chorea, and autoimmune disorders. Biol Psychiatry, 50: 566-578, 2001.

Patwardhan AJ, Eliez S, Warsofsky IS, Glover GH, White CD, Giedd JN, Peterson BS, Rojas DC, Reiss AL. Effects of image orientation on the comparability of pediatric brain volumes using three-dimensional MR data.
J Comp Assist Tomogr 25:452-7, 2001.

Peterson BS, Kane M, Alexander GM, Lacadie C, Skudlarski P, Leung H-C, May J, Gore JC. An event-related functional MRI study comparing interference effects in the Simon and Stroop tasks. Cognitive Brain Res,13:427-440, 2002.

Alexander GM, Packard M, Peterson BS. Sex and spatial position effects on object location memory following intentional learning of object identities. Neuropsychologia, 40:1516-1522, 2002.

Alexander GM, Altemus M, Peterson BS, Wexler B. Replication of a premenstrual decrease in right-ear advantage on language-related dichotic listening tests of cerebral laterality. Neuropsychologia, 40:1293-1299, 2002.

Taylor JR, Morshed SA, Parveen S, Mercadante M, Scahill L, Peterson BS, King, RA, Leckman JF, Lombroso PJ. An animal model of Tourette’s syndrome. Am J Psychiatry 159:657-660, 2002.

Jeong J, Gore JC, Peterson BS. Detecting determinism in a short time series, with an application to the analysis of stationary EEG data. Biol Cybernetics,86:335-342, 2002.

Hampson M, Peterson BS, Skudlarski P, Gatenby C, Gore JC. Detection of functional connectivity using temporal correlations in MR images. Hum Brain Map, 15:247-262, 2002.

JONATHAN POLAN
Polan, H.J., Milano, D., Eljuga, L., & Hofer, M.A. 2002. Development of rats' maternally-directed orienting from birth to day 2. Developmental Psychobiology, 40:81-103.

Polan, H. J., Eljuga, L., Sacks, T., & Hofer, M.A. 2002. Deprivation regulates nipple grasp by P2-3 rat pups in a naturalistic paradigm (abs). Developmental Psychobiology, 41:89.

Eljuga, L., Mathew, E., Hofer, M. A., & Polan, H. J. 2002. Evidence that newborns’ differential responding to maternal cues depends on the first day’s experience (abs). Developmental Psychobiology, 41:76.

MYRNA WEISSMAN
Nomura Y, Warner V, Wickramaratne P. Parents Concordant for Major Depressive Disorder and the Effect of Psychopathology in Offspring. Psychol Med 2001; 31: 1211-1222.
Ritsher JEB, Warner V, Johnson JG, Dohrenwend B. Inter-generational longitudinal study of social class and depression: A test of social causation and social selection models. Brit J Psychiatry 2001; 178: S84-S90.
Weissman MM, Olfson M, Gameroff M, Feder A, Fuentes M: A Comparison of Three Scales for Assessing Social Functioning in Primary Care. Am J Psychiatry 158:460-466, 2001.
Feder A, Olfson M, Gameroff M, Fuentes M, Weissman MM: Medically Unexplained Symptoms in an Urban General Medicine Practice. Psychosomatics 42:261-268, 2001.
Goodwin R, Olfson M, Feder A, Fuentes M, Pilowsky D, Weissman MM: Panic and Suicidal Ideation in Primary Care. Depression and Anxiety 14:244-246, 2001.
Nomura Y, Wickramaratne P, Warner V, Mufson L, Weissman MM. Family Discord, Parental Depression and Psychopathology in Offspring: 10 - Year Follow - up. J Acad Child Adolesc Psychiatry 2002; 41(4): 402-409.
Weissman MM. Juvenile onset major depression includes childhood & adolescent onset depression and may be heterogeneous. Arch Gen Psychiatry 2002, 59(3): 223-234.
Gross R, Olfson M, Gameroff M, Shea S, Feder A, Lantigua R, Weissman MM: Borderline Personality Disorder in Primary Care. Archives of Internal Medicine 162:53-60, 2002.


Faculty - Active Grant Support
William Fifer
“Perinatal Assessment of At-Risk Infants”
Principal Investigator: Fifer, William P. Role: PI (50%)
$398,920 (current year total)
Agency: NICHD Type: R01 (HD 32774) Period: 05/01/99 - 04/30/04
Goals: These studies focus on making cardiorespiratory measurements in groups of human fetuses and newborns who are at risk for developing subsequent neurologic damage and/or Sudden Infant Death Syndrome.

"Fetal Responses to Hypoxia During Development"
Principal Investigator: Stark, Raymond I. Role: Project (8%)
$780,000 (current year total)
Agency NICHD Type 5p50 (HD 13063) Period: 07/01/05
Goals: To conduct basic physiologic, endocrine, and behavioral studies using human premature infants, fetal baboons, and rats to determine responses to and detrimental effects of hypoxia, nutritional variation and nicotine during the perinatal period.

“Fetal Origins of Disease: Markers, Modulators, Mechanisms”
Principal Investigator: Myers, Michael M. Role: Co-Investigator (5%)
$385,964 (current year total)
Agency: NICHD Type: RO1 (HD41326) Period: 08/01/01-06/31/06
Goals: These are animal and human infant studies that focus on cardiovascular and gene expression consequences of variations in early life growth and nutrition.

2R13MH HD58769-03 (Fifer, W.P.) 10/01/00-9/30/05
NIMH
International Society for Developmental Psychobiology Student Travel Grant
R01 HD41326 (Myers, M.M.) 08/01/01-07/31/06
NICHD
Animal and human infant studies to investigate effects of early diets and growth on subsequent cardiovascular function.

2R01 ES08977 (Perera, F.P.) 04/01/02-03/31/07
NIEHS
Environmental Health in a Cohort of Minority Women/Infants
To investigate the impact of pre- and post-natal exposures to air pollutants on fetal growth and early childhood neurobehavioral development.

Primary Mentor on Dianne grant Catherine Grant
Pending Rauh, CEM clinical trials, Towey grant ( co-investigator?)

Myron Hofer
5T32 MH18264 7/1/98 – 6/30/03
NIMH $165,661/year
Research Training – Psychobiological Sciences
Role: Co-Director

This grant provides stipends and associated costs for this post doctoral research
training program.

K-5 MH38632 12/31/97 – 1/1/03
NIMH $100,125/year
The Developmental Effects of Early Maternal Separation

Research Scientist Award to M.A. Hofer. Salary for research (see below) and training roles.

5RO1 MH40430 12/1/96 – 11/30/02
NIMH $176,442/year
The Developmental Effects of Early Maternal Separation
Role: P.I.

To study how the development of an early emotional trait is organized and regulated


Thomas Jessell

Howard Hughes Medical Institute 9/01/02 - 8/31/03 $496,751 (operating expenses)

Molecular Analysis of Vertebrate Neural Development
Research support from HHMI is focused on the inductive interactions that control neural identity in the spinal cord.

RO1 NS33245 09/24/02-08/31/05
NIH $200,000
Control of Motor Neuron Differentiation
To study the mechanisms by which the diversity of different motor neuron subpopulation are generated.

1P50 MH50733
NIH/NIMH (E. Kandel, M.D.) 09/20/94 - 08/31/04 $124,552-Project 1 (Jessell)

Center for Neuroscience Research: Genetic Approaches to Neural Plasticity and Learning
Project 1: The Development of Neural Circuits for a Simple Reflex Behavior: Genetic Analysis of Motor Neuron Identity
This project aims to define the mechanisms whereby distinct classes of motor neurons and interneurons are generated and assembled into functional motor circuits in the spinal cord.


5 P01 CA23767-19 (Competing Renewal)
NIH (Richard Axel, M.D.) 12/1/78 – 07/31/03
$117,443 – Project IV (Jessell)
Proto-Oncogenes: Signaling in Development and Neoplasia
Project IV: Cell Interactions in Motor Neuron Differentiation
The aim of this project will be to focus on the control of motor neuron diversity in the lateral motor column (LMC), a class of motor neurons that innervates target muscles in the limb.

The G. Harold and Leila Y.
Mathers Charitable Foundation
(E. Kandel, M.D.) 9/1/01-08/31/04
$175,000 – Project- Jessell
Molecular Approaches to cognition: The Development and Modification of Internal Representations within the Brain.
The aim of this project is to determine how individual genes contribute to the cellular properties essential for the development and maintenance of cognitive maps.

WELLT066790-C-02-Z
The Welcome Trust 05-01-02-04/30/07
$123.115
Functional Genomics of Neuronal Identity
The aim of this project is to define the molecular cascades and gene networks involved in the determination of two defined neuronal cell types – the motor neuron and the olfactory neuron.

RGP0161/2001
Human Frontier Science Program 4/01/02-03/1/05
$50,000


Functional Analysis of Genetically Defined Interneurons in the Ventral Spinal Cord
The aim of this project is to develop a genetic basis for neuronal classification in the spinal cord.

Project ALS 1/21/02-NA
$100,000
Regulated Gene Expression in Motor Neurons and Neuron Progenitor Cells.
The aim of this proposal is to apply contemporary methods of gene manipulation in the mouse to the study of the origins of ALS and to the design of novel strategies to prevent the death of motor neurons that occurs in ALS and other neurodegenerative disorders.

Michael Myers

“Fetal Origins of Disease: Markers, Modulators, Mechanisms”
Principal Investigator: Myers, Michael M. Role: PI (25%)
$385,964 (current year total)
Agency: NICHD Type: RO1 (HD41326) Period: 08/01/01-06/31/06
Goals: These are animal and human infant studies that focus on cardiovascular and gene expression consequences of variations in early life growth and nutrition.

“Perinatal Assessment of At-Risk Infants”
Principal Investigator: Fifer, William P. Role: Co-Investigator (25%)
$398,920 (current year total)
Agency: NICHD Type: R01 (HD 32774) Period: 05/01/99 -04/30/04
Goals: These studies focus on making cardiorespiratory measurements in groups of human fetuses and newborns who are at risk for developing subsequent neurologic damage and/or Sudden Infant Death Syndrome.

"Fetal Responses to Hypoxia During Development"
Principal Investigator: Stark, Raymond I. Role: Co-Director and
Project Co-Investigator (20%)
$780,000 (current year total)
Agency NICHD Type 5p50 (HD 13063) Period: 07/01/05
Goals: To conduct basic physiologic, endocrine, and behavioral studies using human premature infants, fetal baboons, and rats to determine responses to and detrimental effects of hypoxia, nutritional variation and nicotine during the perinatal period.

“Hostility Reduction and Autonomic Control of the Heart”
Principal Investigator: Sloan, Richard P. Role: Co-Investigator (10%)
$398,710 (current year total)
Agency: NHLBI Type: R01 (HL 63872) Period: 07/01/00 - 06/30/05
Goals: To determine effects of hostility reduction therapy on psychophysiological reactivity in hostility subjects.


Bradley Peterson

1999 NIMH RO1 MH59139R01 FMRI of Impulse Control in Childhood Disorders (B. Peterson, PI)
30% Concurrent Effort. To study the neural basis of impulse control and its dysfunction in childhood neuropsychiatric disorders.
7/1/99-6/30/03 $2,448,553

2000 NIH/NINDS R01 NS27116-09 Randomized Indomethacin GMH/IVH Prevention Trial (L.Ment, PI) To test the hypothesis that the administration of indomethacin protects against long-term structural and functional abnormalities of the developing brain.
20% Concurrent Effort. Dr. Peterson is directing the functional and anatomical MRI components of this project
01/01/00-12/31/04 $5,090,855

2001 Tourette Syndrome Association Neuroimaging Studies of the Amygdala and Hippocampus in TS (B. Peterson, PI) To test the hypothesis that the volumes of amygdala and hippocampus are reduced in individuals who have Tourette syndrome
10% Effort.
7/1/01-6/30/02 $75,000

2001 NIMH R01 Subtyping Schizophrenia with Memory Tests, MRI, and fMRI (B. Wexler, PI)
20% Concurrent Effort. To use neuropsychological tests and MRI to identify neurobiological subtypes of schizophrenia Dr. Peterson is directing the anatomical MRI component of this project. Dr. Peterson is directing the anatomical MRI component of this project.
7/01/01-6/30/06 $1,723,964


Jonathan Polan

“Maternally-directed orienting behaviors of newborn rats”
Principal Investigator: Polan, H. Jonathan
Effort: 75 %
Period: 5/1/98 to 4/30/03
Current year amount: $ 153,337
Agency: NIMH
Grant number: MH01569
Type: K08


Myrna M. Weissman

1 R01 MH63852-01 (Weissman) 07/19/01 to 06/30/06
NIMH $822,786, First Year
Children of Depressed Mothers: a STAR*D Ancillary Study
The overall aim is to study the impact of a reduction of maternal depressive symptoms on children’s psychiatric symptoms and social functioning as an ancillary study to the Sequenced Treatment Alternatives to Relieve Depression study (STAR*D).

2 R01 MH 36197-18 (Weissman) 01/01/99 - 12/31/02
NIH/NIMH $382,178
Children at High and at Low Risk For Depression
Extend assessment to third generation of persons at high risk for MDD to determine if they demonstrate the same clinical features as their parents. Psychophysiologic measures will be employed to seek potential biological markers of a familial diathesis for depressive and anxiety disorders.

1 RO1 MH60912-03 (Weissman) 09/30/99 - 08/31/03
NIH/NIMH $247,683
Genetics of Early-Onset Major Depression
A six-site cooperative project to collect a large sample of subjects with early-onset MDD obtaining clinical data and genetic samples aiming to map genes giving a susceptibility to MDD.

5 R37 MH28274-24S1 (Weissman) 12/01/01 to 06/30/02
NIH/NIMH $86,051
Genetic Studies of Depressive Disorders
This is a project to understand the etiology of panic disorder using a broad range of novel and advanced genetic and epidemiologic approaches.

01 P30 MH60570- 03 (Shaffer) 09/24/99 to 05/31/04
NIH/NIMH $128,451 (Core Only)
Psychotherapy Core, part of Child Psychiatry Intervention Research Center
Provide consultation to investigators on the technology of psychotherapy research and its testing by facilitating training in empirically based treatments and identifying opportunities to test out psychotherapies, both in terms of adapting existing therapies to new uses and implementing new therapies.

(Weissman) 05/01/00 to 04/30/03
NARSAD $100,000 (No cost extension)
A Potential Panic Disorder “Syndrome” Based on Genetic Linkage
NARSAD Distinguished Investigator Award to collect additional information on potential panic disorder syndrome which may include a variety of bladder/kidney problems.

(Weissman) 08/01/01 to 07/31/02
Eli Lilly $150,000
Bipolar Spectrum Disorder in an Urban Primary Care Clinic
A screen of 1,000 patients coming to an Urban Primary Care Clinic for incidence of bipolar spectrum disorder as well as demographic characteristics, mental health treatment, social functioning, and depressive symptoms of patients with and without bipolar spectrum disorder.

2 R01 MH 36197-18 (Weissman) 01/01/99 - 12/31/02
NIH/NIMH $382,178 (Incl. Minority Researcher
supplement)

[NIMH Minority Researcher Supplement to: Adriana Feder]

Children at High and at Low Risk For Depression
Extend assessment to third generation of persons at high risk for MDD to determine if they demonstrate the same clinical features as their parents. Psychophysiologic measures will be employed to seek potential biological markers of a familial diathesis for depressive and anxiety disorders. The supplement extends the high risk design to a Hispanic sample recruited at an urban primary care clinic.

with (Feder) 07/01/02 to 06/30/04
NARSAD $30,000 Year 1
The Neurobiology of Prepubertal Onset Depression
Aims to improve understanding of the neurobiology of prepubertal onset depression and examine the association between prepubertal neurological findings and recurrence of depression into adulthood, lifetime suicidality, and family psychiatric history.


1 R03 MH57813-02 with (Wickramaratne) 12/01/98 to 11/30/2001
NIH/NIMH $50,000 (No Cost Extension)
Epidemiologic Methods in Psychiatric Family Studies

Evaluate existing epidemiologic approaches to the study of familial aggregation in psychiatric disorders and to develop improved approaches to the design and analyses of such studies.


Total Yearly Outside Support for All Faculty: $ 9,458,000/year
(direct costs only)


SACKLER AWARDEE - FINAL REPORT


Maternal Depression: Fetal and Infant Neurobehavior

Catherine E. Monk, PhD • Columbia University, New York, NY

July 2002

In June, 2000, I was granted a Sackler Institute Award to support the study of fetal characteristics associated with maternal mood disorder. Our goal was to begin to establish a program of research exploring the potential affects of stress, anxiety, and depression, on fetal development. Our work is focused on (1) finding evidence for the hypothesis that how women function emotionally does affect the fetus and then (2) pursuing more specifically the influence of depressed women’s altered physiology on fetal development. With the later goal, we aim someday to discover in utero markers for future risk for Major Depressive Disorder (MDD). I am happy to report that the project progressed as planned. Specifically, we generated important data now in press with the Journal of Developmental and Behavioral Pediatrics showing the acute transmission of women’s stress–based physiology to the fetus. In addition, we generated preliminary data that was instrumental in our securing federal funding for future related studies. In what follows, I describe more fully the work accomplished through the support of this award.

Paper in press showing the women’s psychologically–based mood affecting the fetus
In this paper, entitled, The effects of women’s stress–elicited physiological activity and chronic anxiety on fetal heart rate, we examined the effects of pregnant women’s acute stress reactivity and chronic anxiety on fetal heart rate. We found that fetal heart rate changes during women’s recovery from a stressful cognitive task performed on a computer were associated with the women’s concurrently–collected heart rate and blood pressure changes (r=.63, p<.05). Fetal heart rate changes during recovery, as well as during women’s exposure to the stressful task, were correlated with their mothers’ trait anxiety scores (r=.39, p<.05 and r=–.52, p<.01, respectively). Finally, a combination of measures of women’s cardiovascular activity during recovery and trait anxiety scores accounted for two–thirds of the variance in fetal heart rate changes during the same recovery period (R2=.69, p<.001). Such findings raise the possibility that cumulative exposure to particular patterns of emotion–induced physiological activity over the course of gestation may influence fetal development. Moreover, our results also indicate an influence of women’s anxiety on fetal HR. This association suggests the possibility that there may be a trait–like difference in fetal HR activity that is associated with a chronic emotional state in pregnant women. Although this could be an inherited trait, the findings here indicating the possible acute transmission of women’s stress–elicited cardiovascular activity to the fetus support the conceptualization that throughout pregnancy, qualities of the in utero environment— such as the repetition of anxiety–based alterations in the women’s physiological activity—may influence fetal development and contribute to the emergence of individual differences in the fetus. Findings from this report are some of the first ever to determine a ‘real time’ association between fetal HR and women’s psychologically–induced changes in cardiovascular activity. They are consistent with a model in which differences in biobehavioral development may be, in part, shaped in utero. Longitudinal studies focusing on fetal, infancy, and childhood periods will help determine whether fetal characteristics associated with women’s emotional states during pregnancy are transitory or, in fact, indicative of a process leading to trait–like characteristics that have implications for the child’s long–term health and development.

Moreover, these findings demonstrating ‘real time’ effects of women’s emotion–based physiological changes on the fetus are of central importance as we pursue our research goal to determine if depression during pregnancy affects the fetus, and fetal development, via similar physiological pathways. These results thus support our research goal as we investigate this secondary agenda.

Preliminary data used to garner federal funding:
Subject Enrollment: We recruited 126 pregnant women for the study. Of these 126 women, so far 94 have completed the first study session. We have run newborn assessments on the 69 babies that have so far been born. Approximately 41% of our subjects are diagnosed with depression, and anxiety disorder, or a co–morbid mood disorder. Although our final analyses are yet to be completed (and some subjects are still moving through the longitudinal protocol) as can be seen from the results described below, we have met our goal of recruiting pregnant women with psychiatric diagnoses as well as healthy controls.

Preliminary Data:
At the outset of our research, we hypothesized that (1) maternal depression will be associated with fetal physiological dysregulation as indexed by a greater heart rate (HR) increase during women’s exposure to a stressor and (2) that newborns of women depressed during pregnancy will show altered physiology as measured by HR changes during an orthostatic challenge.

Our findings support our hypotheses. Specifically, preliminary analyses suggest that, as predicted, during women’s exposure to a laboratory “stressor”, fetuses of depressed women show greater HR reactivity (see bar graph of fetal HR activity related to women’s diagnostic status below). Moreover, we now have evidence that alterations in HR patterns are evident in the newborns of women who had mood disorders during pregnancy (see bar graph below). Specifically, new data indicates that newborns of women who were depressed during pregnancy, or had anxiety disorders, do not have as large a HR decrease to a head–down tilt challenge compared to babies born to healthy controls. These data indicate that we can detect differences in fetal behavior related to women’s mood during pregnancy. In conjunction with the newborn study, they also suggest that the alterations in HR patterns evidenced in the fetal period related to women’s mood disorder are not merely transitory, nor dependent on qualities of the in utero environment. The data are consistent with the hypothesis that mood–based alterations in women’s physiology during pregnancy may influence the developing child’s neurobehavioral substrate,—the building blocks of future emotion regulation—with implications for the child’s future risk for mental illness. Moreover, with this work, we believe we may be in the early stages of discovering novel and very early markers for a vulnerability to affective disorder linked to a shared genetic predisposition of the mother and infant. Identification of early markers for risk for mental illness will lead to earlier therapeutic interventions, and ultimately, to greater prevention of psychiatric illness.

 

 

 

Sample list of presentations & publications supported by the Sackler Award

Monk, C., Sloan, R. P., Myers, M. M, Ellman, L. & Fifer, W.P., The effects of women’s stress–elicited physiological activity and chronic anxiety on fetal heart rate In Press. Development and Behavioral Pediatrics.

Fifer, W.P., Monk, C.M. and Grose-Fifer. Prenatal Development and Risk. In G. Bremner and A. Fogel (Eds.), Blackwell Handbook of Infant Development. Oxford, UK: Blackwell. (2001).

Monk, C., Fifer, W.P., Sloan, R. P., Myers, M. M. Newborn infants exposed to maternal psychiatric illness during pregnancy have diminished responses to downward tiling [Abstract] Psychosomatic Medicine; (2002) 64: 89. To be presented at the annual meeting, American Psychosomatic Meeting, March 13-20, 2002, Barcelona, Spain.

Monk, C., Fifer, W.P., Sloan, R. P., Myers, M. M. Maternal depression influences patterns of fetal reactivity. Presentation made at the Society for Research in Child Development, April 18–22, 2001. Minneapolis, Minnesota


Other sources of funding
On October 1, 2001, I received a 5–year grant from the National Institutes of Mental Health. The title of the project, “Maternal Psychopathology: Fetal and Infant Neurobehavior” is closely related to the Sackler Award as the project builds on that initial work. Moreover, funding from the Sackler Award was instrumental in my obtaining this federal support. Without financial backing from the Sackler Award, we never would have been able to generate the pilot data to compete for such an award. Having used the Sackler grant as seed money to successfully win federal funding, we now are in a position to build a research program focused on detecting the earliest influences on future risk for mental illness. We believe that these studies ultimately will lead to the establishment of clinical interventions that will benefit pregnant women and their future children.

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